Figure 2

AICAR treatment inhibits LPS + Aβ-stimulated iNOS gene expression and nitric oxide release in glial cells. Cell cultures were pre-incubated with 1 mM AICAR following stimulation by LPS ± Aβ peptides (1–40) or (1–42) in concentrations as indicated. The corresponding reverse peptide (40–1) in lane 3 and 9 served as a positive control in this assay. The production of NO (top) and expression of iNOS, COX-2, and MnSOD was determined in cell lysates, 18 h following treatment, by immunoblot analysis (bottom). Experiments were performed in triplicate and data are means (±SEM). P < 0.05 compared to relative control value was considered significant. However, P value for histograms in lane 8 and 9 (* and **) not significant.