Figure 3

AICAR inhibits LPS- and Aβ (25–35) peptide-induced cytokine production in glial cells stimulated with 125 ng/ml LPS ± 7.5 μM Aβ peptide (25–35). After 18 h of incubation, concentrations of TNF-α, IL-1β, and IL-6 released into the culture medium were measured using ELISA. Fig. 3A. Shows dose-response curves, using LPS and various concentrations of Aβ (25–35) peptide in stimulating cytokine release in glial cells. Fig. 3B shows a dose-response inhibition of cytokine release with various concentrations of AICAR (0.25 to 1 mM) following stimulation with LPS + Aβ (25–35) peptide.