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Figure 11 | Journal of Neuroinflammation

Figure 11

From: A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices

Figure 11

Neurons stained with DCX and astrocytes with GFAP. (A) DCX-positive cells in the dentate gyrus of P7 hippocampus slice treated for 24 h with LPS (5 μg/ml) and (B) LPS and TSA (20 nM) at 7 DIV. The treatments had no visible effect on morphology (inserts in A & B, scale bars equal 50 μm), staining intensities or number of labeled neurons. (C) Astrocytes in the CA-area of control slice and (D) LPS treated slice after 14 DIV. Similar long processes were visible in both groups as at 5 DIV (Fig. 9C). Scale bars: A and B = 100 μm, C and D = 50 μm.

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