Figure 2
Immunization of animals at 12–16 months with MAPAβ1–33 decreases thioflavine and anti CD45 reactivity. A. Representative photomicrographs of thioflavine (green) or CD45 (brown) staining of brain sections from 16 months Tg2576 untreated (control), or injected with adjuvant alone (SCFA) or MAPAβ1–33 (MAP). Scale bar 100 microns (upper panel) and 50 microns (lower panel). B. Image analysis of thioflavine staining or CD45 immunoreactivity in cortex and hippocampus of animals untreated or immunized at 12–16 months. Mean of each animal is the average of two sections (stained in two independent assays, each of which contained animals from each treatment group) in which most to all the area of study was analyzed (4–8 images per section). Bars represent mean +/- SD of n mice per group. Thioflavine: Control n = 6, SCFA n = 6, MAP n = 5, *p < 0.02; CD45: Control n = 5, SCFA n = 7, MAP n = 5, * p < 0.04.