Figure 2
Selective labelling of SVMs with rhodamine dyes. (A) RHO+ cells are microglia. Transmission electron microscopy demonstrates dye-laden inclusions (white arrows) in a SVM. n, nucleus. (B) Immunohistochemistry for F4/80 (top) and histochemistry for lectin IB4 (bottom) demonstrate double-labelled periventricular cells, white arrows. (C) Time-lapse confocal microscopy in live brain slices demonstrates SVM (white arrow) extending (time 0' and 9') and retracting (time 4.5' and 13.5') a process toward ependymal debris (yellow star) highly suggestive of phagocytosis. See also Video 1. lv, lateral ventricle; p, parenchyma. (D) Neuraminidase injection following sublethal ependymal labelling similarly results in RHO+ SVMs (black arrows). e, ependyma; lv, lateral ventricle; p, parenchyma. Left panels, hematoxylin; Right panels, RHO fluorescence overlaid on hematoxylin.