Figure 2

Cytokine secretion following LPS stimulation. Mouse cerebral primary astrocyte cultures were incubated in serum-free medium with 100 ng/ml LPS or PBS for 12 hr, medium collected, and IL-1β, IL-6 and TNF-α concentrations determined by ELISA. All cytokines were below the limit of detection in PBS-exposed cultures. Data are mean ± SEM (n = 4 to 8 separate cultures per group). One-way ANOVA showed P < 0.05 for all three cytokines. *P < 0.05, ^{^}P < 0.01, or ^#P < 0.001 for Bonferroni-corrected posttests for LPS-incubated TR APOE3 or TR APOE4 vs. TR APOE2; ⁺P < 0.01 for TR APOE4 vs. TR APOE3.