Figure 3
From: Apolipoprotein E-specific innate immune response in astrocytes from targeted replacement mice
NF-κB activity following LPS stimulation. NF-κB p50 and p65 subunit activity was determined in nuclear extracts from mouse cerebral primary astrocyte cultures exposed to vehicle (PBS) or LPS for 12 hr using an NF-κB transcription factor assay kit (Chemicon). Data are expressed as average relative light units (RLU) ± SEM (n = 4 for each group). Two-way ANOVA for p50 data had P < 0.0001 for TR APOE and vehicle vs. LPS, but P > 0.05 for interaction between these terms. Two-way ANOVA for p60 data had P < 0.05 for TR APOE and P < 0.0001 for vehicle vs. LPS, but P > 0.05 for interaction between these terms. *P < 0.01 for Bonferroni-corrected posttests for LPS-exposed TR APOE3 or TR APOE4 vs. TR APOE2.