Figure 4From: Primary glia expressing the G93A-SOD1 mutation present a neuroinflammatory phenotype and provide a cellular system for studies of glial inflammationBasal and cytokine-stimulated protein carbonylation is increased in G93A-SOD1 astrocyte cultures. Cells were stimulated for 48 hours with 50 U/mL IFNγ plus 40 ng/mL TNFα, lysed in the presence or absence of biotin-LC-hydrazide (+ or - label as indicated), blotted onto a PVDF membrane and probed with streptavidin-conjugated horseradish peroxidase.Back to article page