Figure 3

Immunoperoxidase histochemistry analyses of paraffin-wax sections to assess the expression of complement regulatory proteins (CRegs) in control and meningitis cases. Rehydrated paraffin wax sections of human choroid plexus in normal and meningitis cases immunostained with antisera to inflammatory cells and to membrane regulators proteins. Original magnification, ×400. Panel A: Choroid plexus staining data: a and b, Rabbit anti-GFAP. c and d, Rabbit anti-HLA II staining only Kolmer cells (arrow). No differences are noticed between normal and meningitis cases. e and f, Rabbit anti-CD55. Choroid plexus epithelium in normal cases is weakly stained (e) but more strongly in meningitis cases (f). Erythrocytes in f are stained for CD55 (arrow). g and h, Rabbit anti-CD46. Epithelium is weakly stained in normal cases (g) but strongly in meningitis epithelia, while infiltrating PMN (arrow) are strongly CD46+ (h). i and j, Rabbit anti-CD35. Weak staining is detected on Kolmer cells in normal sections (i) but a strong staining is noticed in meningitis epithelia and infiltrating PMNs (j). k and l, Rabbit anti-CD59. Normal choroid plexus epithelia are stained for CD59 (k) and with a stronger staining in meningitis (l). Panel B: same as above but assessing the expression of CRegs on ependymal cells. Note that erythrocytes (Er) within blood vessels are strongly stained for CD55 (f).