Figure 1

Schematic maps of animal experiments. The course of tMCAo and sham surgery is shown in 1A. Note that all animals were anesthetized nearly 160 minutes while they underwent surgery. Parecoxib (10 mg/kg BW) or isotonic saline was administered IP within the first five minutes after the start of tMCAo or sham. 1B illustrates steps in the qRT-PCR part of the study. Only half of the animals subjected to tMCAo underwent MRI prior to euthanasia. 1C shows complete drug administration plan in the neurogenesis part. Buprenorphine (0.03 mg/kg BW) was given IM as a pain killer for the first two days twice daily. Parecoxib (10 mg/kg BW) or isotonic saline was injected IP twice daily throughout the investigation period. Finally, BrdU (50 mg/kg BW) was administered IP at 7 am and 3 pm on Day 4 to Day 7. Six tMCAo animals randomized to parecoxib treatment and four SHRs receiving isotonic saline commenced MRI on both Day 2 and Day 8.