Table 2 IFN-γ treated neurons show increased sensitivity to HuPrP82-146. Neurons treated with 1 ng/ml IFN-γ for 24 hours prior to the addition of neurotoxins as shown. Cell survival was determined 24 hours later using the WST-1 test. Each value is the mean percentage cell survival ± SD from triplicate experiments repeated 3 times (9 observations). ** = Neuronal survival significantly less than untreated neurons incubated with HuPrP82-146 (p < 0.05).
From: Interferon-γ increases neuronal death in response to amyloid-β1-42
Neuronal survival (%) | |||
|---|---|---|---|
conc | Control | IFN-γ | |
HuPrP82-146 (μM) | 40 | 48 ± 6 | 12 ± 7** |
10 | 64 ± 5 | 22 ± 8** | |
2.5 | 92 ± 9 | 41 ± 9** | |
Staurosporine (ng/ml) | 20 | 37 ± 4 | 40 ± 6 |
5 | 78 ± 6 | 72 ± 8 | |
1.25 | 92 ± 3 | 95 ± 4 | |
Hydrogen peroxide (μM) | 1 | 22 ± 5 | 18 ± 7 |
0.25 | 58 ± 7 | 55 ± 6 | |
0.06 | 87 ± 6 | 89 ± 9 | |