Figure 5
From: Interleukin-1β and anaphylatoxins exert a synergistic effect on NGF expression by astrocytes
Expression of neurotrophic factors by rat primary astrocytes stimulated by MAP-C3a or MAP-C5a (10-8M). Autoradiogramm of neurotrophin mRNA expression by unstimulated rat primary astrocytes is shown on the left panel: Lane 1 represents the Multi-Probe Template Set not treated with RNases and Lane 2 the neurotrophin mRNA expression by unstimulated rat primary astrocytes. Note that each probe band (Lane 1) migrates slower than its protected band (Lane 2); this is due to flanking sequences in the probe that are not protected by mRNA. RNA was then extracted after 2 h, 4 h, 6 h, 12 h and 24 h of stimulation and analyzed using L-32 and GAPDH as internal standards. On the right panel, results are represented as histograms and expressed as relative fold increase over control (NS = non-stimulated cells). This is a representative graph of n = 3 independent experiments.