Membrane fraction analysis of the effect of SN on cytosolic p47phoxprotein translocation. HAPI cells were pretreated with vehicle or SN (10-14 M) for 30 min, followed by LPS treatment for 10 min. Membrane fractions were isolated to perform western blot analysis, using gp91phoxas an internal membrane control. Each experiment was performed three times. *P < 0.05, compared with the LPS-treated cultures.