Effects of a single dose of ethanol exposure on LPS-induced liver, serum and brain TNFα production. Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) for one day. Mice were injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Liver, serum and brain samples were collected at 1 hr post LPS treatment. Analysis of TNFα and MCP-1 protein was conducted by ELISA. (A) LPS and ETOH-LPS rapidly increased liver, serum and brain TNFα protein. (B) MCP-1 protein in liver, serum and brain was increased after LPS treatment alone or combined LPS and ethanol treatments. The results are the means ± SEM (n = 6 per group). * P < 0.05, ** P < 0.01, compared with the saline controls.