Effects of 10 daily doses of ethanol exposure on LPS-induced liver, serum and brain TNFα and MCP-1 production. Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) daily for 10 days and injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Liver, serum and brain samples were collected 1 hr post LPS treatment. TNFα and MCP-1 protein was determined by ELISA. (A) In 10-day ethanol pre-treated group, LPS significantly increased liver, serum and brain TNFα protein compared with LPS alone group. (B) Exposure to 10 daily doses of ethanol resulted in a significant increase in MCP-1 protein in liver, serum and brain. The results are the means ± SEM of two experiments performed with 6 mice each group. * P < 0.05, ** P < 0.01, compared with the saline controls. # P < 0.05, ## P < 0.01, compared with LPS-treated group.