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Table 3 Ten daily doses of ethanol and LPS induced changes in gene expression in brain and liver at 1 week

From: Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment

Gene Control ETOH LPS 0.03 LPS 0.3 LPS 3 LPS 0.03 + ETOH LPS 0.3 + ETOH LPS 3 + ETOH
Brain         
TNFβ 100 ± 19 109 ± 17 113 ± 19 78 ± 21* 225 ± 21** 134 ± 6 172 ± 21* 307 ± 47**
MCP-1 100 ± 7 138 ± 5** 109 ± 8 122 ± 5* 163 ± 24* 139 ± 10* 150 ± 5**# 147 ± 13*
IL-1β 100 ± 8 114 ± 9 137 ± 10* 132 ± 19 132 ± 13 109 ± 9 108 ± 10 149 ± 23
gp91phox 100 ± 12 133 ± 4* 102 ± 14 151 ± 9* 156 ± 17* 166 ± 21*## 182 ± 18** 236 ± 30**#
p67phox 100 ± 6 133 ± 12* 113 ± 11 153 ± 6** 164 ± 15** 112 ± 10 136 ± 19 155 ± 22*
DBI 100 ± 16 199 ± 28* 187 ± 15** 181 ± 7** 217 ± 21** 219 ± 7** 239 ± 13**# 188 ± 43
IL-10 100 ± 28 ND ND ND ND ND ND ND
Liver         
TNFβ 100 ± 11 116 ± 11 90 ± 9 110 ± 17 107 ± 16 110 ± 7 95 ± 10 115 ± 11
MCP-1 100 ± 6 110 ± 11 86 ± 16 100 ± 12 87 ± 14 102 ± 4 109 ± 9 104 ± 7
IL-1β 100 ± 21 93 ± 19 84 ± 18 63 ± 8 59 ± 6 40 ± 5* 39 ± 3* 43 ± 5*
IL-10 100 ± 21 243 ± 33* 222 ± 25*## 485 ± 92** 212 ± 49 427 ± 29** 312 ± 57* 281 ± 79
  1. ND = not detected. Ten daily doses of ethanol and LPS induced changes in gene expression in brain and liver at 1 week. Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) daily for 10 days and injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Brain and liver samples were collected 1 week post LPS treatment. Gene expression of proinflammatory and anti-inflammatory factors was measured by real-time PCR. The results are the means ± SEM (n = 6 per group). * P < 0.05, ** P < 0.01, compared with the saline controls. # P < 0.05, ## P < 0.01, compared with LPS-treated group.