Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment

Table 3 Ten daily doses of ethanol and LPS induced changes in gene expression in brain and liver at 1 week

Gene	Control	ETOH	LPS 0.03	LPS 0.3	LPS 3	LPS 0.03 + ETOH	LPS 0.3 + ETOH	LPS 3 + ETOH
Brain
TNFβ	100 ± 19	109 ± 17	113 ± 19	78 ± 21*	225 ± 21**	134 ± 6	172 ± 21*	307 ± 47**
MCP-1	100 ± 7	138 ± 5**	109 ± 8	122 ± 5*	163 ± 24*	139 ± 10*	150 ± 5**#	147 ± 13*
IL-1β	100 ± 8	114 ± 9	137 ± 10*	132 ± 19	132 ± 13	109 ± 9	108 ± 10	149 ± 23
gp91^phox	100 ± 12	133 ± 4*	102 ± 14	151 ± 9*	156 ± 17*	166 ± 21*##	182 ± 18**	236 ± 30**#
p67^phox	100 ± 6	133 ± 12*	113 ± 11	153 ± 6**	164 ± 15**	112 ± 10	136 ± 19	155 ± 22*
DBI	100 ± 16	199 ± 28*	187 ± 15**	181 ± 7**	217 ± 21**	219 ± 7**	239 ± 13**#	188 ± 43
IL-10	100 ± 28	ND	ND	ND	ND	ND	ND	ND
Liver
TNFβ	100 ± 11	116 ± 11	90 ± 9	110 ± 17	107 ± 16	110 ± 7	95 ± 10	115 ± 11
MCP-1	100 ± 6	110 ± 11	86 ± 16	100 ± 12	87 ± 14	102 ± 4	109 ± 9	104 ± 7
IL-1β	100 ± 21	93 ± 19	84 ± 18	63 ± 8	59 ± 6	40 ± 5*	39 ± 3*	43 ± 5*
IL-10	100 ± 21	243 ± 33*	222 ± 25*##	485 ± 92**	212 ± 49	427 ± 29**	312 ± 57*	281 ± 79

ND = not detected. Ten daily doses of ethanol and LPS induced changes in gene expression in brain and liver at 1 week. Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) daily for 10 days and injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Brain and liver samples were collected 1 week post LPS treatment. Gene expression of proinflammatory and anti-inflammatory factors was measured by real-time PCR. The results are the means ± SEM (n = 6 per group). * P < 0.05, ** P < 0.01, compared with the saline controls. ^# P < 0.05, ^## P < 0.01, compared with LPS-treated group.

ISSN: 1742-2094