Double immunostaining of IgG extravasation and the expression of occludin. Cholesterol-enriched diet markedly decreased the immunopositive staining of occludin (green) at the sites where IgG extravasation (red) was present. These effects were blocked by chronic ingestion of caffeine. Representative images taken from 2 rabbits in each group with 6 sections from each animal are shown. Bar = 20 μm.