Figure 4

CDDO-Me suppresses basal and inflammation-induced gene expression. BV2 microglia cultures were pre-incubated with 100 nM CDDO-Me for 17 hrs before a 4-hr stimulation with 10 ng/mL LPS in serum-free medium. (A) Scatterplot of baseline inflammatory gene expression in unstimulated BV2 cells in the presence of DMSO vehicle (0.1%) versus unstimulated cells in the presence of 100 nM CDDO-Me. (B) Unstimulated cells in the presence of DMSO vehicle versus cells stimulated with LPS (10 ng/mL) in the presence of DMSO vehicle, or (C) cells stimulated with LPS in presence of DMSO vehicle versus cells stimulated with LPS in the presence of 10 nM CDDO-Me plus. Data analyses were performed using the Scatter Plot data analysis tool in the SuperArray GEArray Analysis Suite online (See Methods). Genes shown with a red plus (+) sign were up-regulated and genes shown with a green minus (-) sign were down-regulated in the treatment condition, which is plotted on the Y-axis relative to the control condition plotted on the X-axis.