SLPI enhances the differentiation of oligodendroglial cells. Differentiation and cell death analysis of NSC treated with indicated amounts of SLPI. NSC mounted on microscope slides were cultivated for three days with the specified amounts of SLPI in growth medium and subsequently for seven days in differentiation medium. Cells were stained for cell specific markers and counted. The shown values are representative for three independent experiments. A: proportion of GFAP positive astroglial cells (± std dev), B: proportion of βIII-tubulin positive neuronal cells (± std dev), C: proportion of GalC expressing oligodendrocytes (± std dev). *: p < 0.05 (according to a One Way ANOVA analysis of variance (Holm-Sidak method)). D: To detect dying cells, 50 μg/ml propidium iodide was added to the culture medium. After ten minutes of incubation. The fraction of PI positive cells and the total cell number was determined by counting. Presentation of the proportion of dying cells and total cell number (± std dev).