FLZ attenuates LPS-induced microglia activation. (A): Neuronal-glial cultures were pretreated with 10 μM FLZ for 1 h followed by 2 ng/ml LPS; 24 h later, activation of microglia was assessed by OX-42 immunohistochemistry. Representative results are illustrated. (B): HAPI microglia cells were pretreated with 10 μM FLZ for 1 h followed by stimulation with 10 ng/ml LPS for 24 h. Expression of MHC class II antigen was detected by flow cytometry. The cells were analyzed on a FACS Calibur, and the MFI of experimental groups was determined by subtracting the MFI of the isotype control from the MFI of each group. The data are expressed as mean ± S.E.M. for three independent experiments, each performed with triplicate samples. ## p < 0.01 compared with vehicle-treated control group; * p < 0.05 compared to LPS treatment group. Scale bar, 50 μm.