Figure 3

Colocalization of activated microglial cells with Aβ plaques and IL-1β immunoreactivity in the TgAPPsw mice with reduced GRK5. Panels A-C, IF staining of Aβ⁺ plaques (A, red) and surrounding CD45⁺ microglial cells (B, green), as well as their merged view (C) in the double mice. Scale bar: 50 μm for panels A-C. Panels D-F, IF staining of Aβ⁺ plaques (D, red) and surrounding CD11b⁺ microglial cells (E, green), as well as their merged view (F) in the double mice. Scale bar: 50 μm for panels D-F. Panel G, an example of merged view for CD45 (green) and CD11b/c (clone OX42, red) co-staining of the microglial cells. The image showed that, at least in this particular experimental paradigm, the CD45 antibody stained more specifically for the microglial cell profiles; while the OX42 antibody, in addition to its positive staining of the microglial cells, also non-specifically decorated the plaques. Scale bar: 45 μm. Panel H, an example of merged view for Aβ⁺ plaques (red) and surrounding IL-1β immunoreactivity (green) in the double mice. Scale bar: 30 μm. Panel I, colocalization of CD45⁺ microglial cells (green) with IL-1β immunoreactivity (red) in the double mice. Scale bar: 30 μm. Blue indicates reference DAPI staining of nuclei.