Inhibition of TLR2-mediated, Yps3p-induced NF-κB activation in 293T-mTLR2 cells by VV proteins. Plasmids carrying the open-reading frames of A46R, A52R, K1L, or N1L were co-transfected along with pNiFty2-Luc, cells were incubated overnight at 37°C and treated with Yps3p for 5 h. Cells were then harvested and the amount of luciferase produced was quantified using bright glow substrate. Data are presented as mean ± SD of triplicate samples and are representative of three independent experiments. Statistical analysis was performed by student's t test. *P < 0.05; **P < 0.01.