Lower IL-1β production with a TLR7/8 agonist in the ASD test group without LPS pre-treatment. In all experiments shown in Figures 1, 2, 3, 4, 5 and 6, PBMCs were incubated overnight with TLR2/6, TLR4 or TLR7/8 agonists, and cytokine production was examined by ELISA. The results were expressed using a boxplot: The "I" bar marks the range of TGF-β and IL-10 levels and the thick black line marks the median. The box illustrates where the 'interquartile range' falls. 'Outliers' are marked separately as 'o'. IL-1β production with a TLR7/8 agonist was lower in the ASD test group than in the ASD case controls.