Rat microglial primary cultured cells were exposed for 24 h to 300 nM BK to increase B
R expression. Then, they were exposed to TOPRO-3, a specific fluorescent dye for DNA (A), to anti-IBA-1, a specific antibody against microglia (B) and BdABK to stain B1R (C). Colocalization of the three markers is shown in panel D. TOPRO-3 dye is blue (ext: 642 nm/em: 661 nm), anti-IBA-1 dye is red (ext: 550 nm/em: 570 nm) and BdABK dye is green (ext: 505 nm/em: 515 nm). Scale bar = 31.75 μm. Pictures presented are representative of 4 cultured cells samples from 4 different animals.