STZ-treated spinal cord was exposed to anti-CGRP, a selective antibody of sensory C fibers (A). TRPV1 was labeled with anti-TRPV1 another marker of sensory C fibers (B). The B1R was stained with the selective fluorescent agonist, BdABK (C). Colocalization of the three markers is shown in panel D. Anti-CGRP dye is blue (ext: 650 nm/em: 680 nm), anti-TRPV1 dye is red (ext: 550 nm/em: 570 nm), and BdABK dye is green (ext: 505 nm/em: 515 nm). Scale bar = 19.21 μm. Pictures presented are representative of a minimum of 4 sections per rat from 4 different animals.