Figure 3
From: Modulation of inducible nitric oxide synthase expression by sumoylation
Effect of increasing SRG expression on promoter activity. C6 cells stably transfected with pNFκB (4 × NFκB binding-element) or pGL3-2.2 (2.2 kB NOS2 promoter sequence) luciferase reporter plasmids were transiently transfected with SUMO-1, Ubc9, or SENP1 expression plasmids or with vector only (pcDNA3). After 40 hours cells were treated with LPS for 4 hours and luciferase activity measured. The data shows relative luciferase activity of treated versus untreated cells, and is the mean ± sd of 3 independent experiments. *, p < 0.05; **, p < 0.01 vs vector (unpaired t-test).