Figure 4
From: Modulation of inducible nitric oxide synthase expression by sumoylation
Sumoylation of C/EBPβ in astrocytes. Primary rat astrocytes were co-transfected with YFP-SUMO-1 and CFP-C/EBPβ expression plasmids. After 24 hr the cells were fixed, and cells co-expressing YFP-SUMO-1 and CFP-C/EBPβ were subjected to FRET analysis. (A) Representative image of CFP and YFP fluorescence after linear un-mixing of emission spectra and merged image showing the region of interest (ROI) analyzed. (B) FRET data after acceptor photobleaching of the ROI indicated ("1") in panel A, showing an increase in CFP fluorescence coincident with a decrease in YFP fluorescence. (C) CFP fluorescence of ROIs measured in 7 different cells before (open bars) and after (filled bars) acceptor photobleaching. "Ave" mean ± s.e (n = 7) of CFP fluorescence. *, p < 0.05 after versus before, non parametric paired T-test.