Figure 1

Effects of IFN-γ, LPS and GW 501516 on GFAP, TNF-α, IL-6, and iNOS mRNA expression. GW 501516 down-regulated GFAP mRNA expression in control cultures and in cultures treated with the inflammatory agents (A). GW 501516 decreased the up-regulation of TNF-α induced by the inflammatory agents. GW 501516 up-regulated IL-6 expression in control cultures and in cultures treated with the inflammatory agents (C), and it decreased the IFN-γ-induced up-regluation of iNOS expression (D). Cultures received GW 501516 (5 μM) 18 hours before the addition of the inflammatory agents, and again together with INF-γ (50 U/ml) and LPS (5 μg/ml). Cultures were harvested 48 hours after the inflammatory treatment. Values are expressed as fold change relative to the untreated control cultures (= 1), each value being the mean of 7 replicate cultures. Results were statistically evaluated for significance by the Kruskal-Wallis test followed by the Mann-Whitney test. (* P < 0.05, **P < 0.01,***P < 0.001 compared with untreated control cultures; °°P < 0.01, °°°P < 0.001 compared with cultures not treated with GW 501516).