Figure 6
From: Unique aspects of transcriptional regulation in neurons – nuances in NFκB and Sp1-related factors
ApoE declines with Sp1 during neuronal differentiation but is induced by glutamate. A. NTera2 cells were harvested undifferentiated (U) or after incubation as neurospheres in retinoic acid for 14 days (RA). Subsequent to RA treatment, additional cultures underwent selection by incubation with a cocktail of mitotic inhibitors (MI) for 7 days. Equal amounts of protein were resolved by SDS-PAGE, and the levels of ApoE, Sp1, and Sp4 were assessed by western blot analysis. B. Differentiated NTera2 cells were untreated or treated with 20 μM glutamate for 20 h, then levels of ApoE and actin were assessed by western blot.