A. Schematic representation of the experimental design. Subchronic microinjections into the right substantia nigra and striatum were processed at 13, 17, 18 and 19 weeks of age. Mice (four groups) were microinjected with either DMSO/PBS (vehicle) or three concentrations of PGJ2/PBS (3.4 μg, 6.7 μg or 16.7 μg) per injection. All animals were perfused intracardially for pathohistological examinations at 23 weeks of age, thus four weeks after the last injection. Behavioral tests were performed four weeks after the last microinjection. B – Site of injection. Immunofluorescence analysis with anti-tyrosine hydroxylase (red, dopaminergic neuronal marker) and anti-IBA1 (green, activated microglia marker) antibodies revealed nonspecific damage at the site of injection in a mouse infused with DMSO/PBS (vehicle). The star indicates the site of injection. Scale bar = 100 μm.