Figure 6From: Absence of the complement regulatory molecule CD59a leads to exacerbated neuropathology after traumatic brain injury in miceRegulation of intracerebral protein levels of apoptotic mediators. Western blot analysis was performed in murine brain homogenates (injured left hemispheres) using mouse-specific primary antibodies and detection by a non-radioactive chemoluminescence assay, as described in the methods section. Equal protein amounts (60 μg per lane) were loaded on SDS-PAGE and consistent loading and blotting was confirmed by ponceau staining (not shown) and control blotting with β-actin. The Western blots shown in this are representative for n = 3 per experiment. No significant differences were seen between the groups at a qualitative (figure) or quantitative level (not shown). TBI, traumatic brain injury; WT, wild-type.Back to article page