Regulation of intracerebral protein levels of apoptotic mediators. Western blot analysis was performed in murine brain homogenates (injured left hemispheres) using mouse-specific primary antibodies and detection by a non-radioactive chemoluminescence assay, as described in the methods section. Equal protein amounts (60 μg per lane) were loaded on SDS-PAGE and consistent loading and blotting was confirmed by ponceau staining (not shown) and control blotting with β-actin. The Western blots shown in this are representative for n = 3 per experiment. No significant differences were seen between the groups at a qualitative (figure) or quantitative level (not shown). TBI, traumatic brain injury; WT, wild-type.