Effect of IR injury and DEDA on Ca2+ dependent PLA2 activity, mRNA and protein expression of sPLA2 in brain tissue at 24 hours of reperfusion after 60 min MCAO. IR induced activity of calcium-dependent PLA2 was measured in both ipsilateral as well as contralateral hemispheres. Treatment with DEDA reduced the PLA2 activity (A). However, treatment with DEDA did not alter IR-induced mRNA expression of sPLA2 measured at 4 hours of reperfusion (B). Representative photomicrograph of protein expression of sPLA2 at 24 hour reperfusion (C). Data represented as means ± SD of triplicate determinations (n = 5). * p < 0.05 vs. Sham, ** p < 0.01 vs. Veh-contra, +++ p < 0.001 vs. Veh-Ipsi.