A-B: c-Fos immunoreactivity within the DVC. Representative coronal sections of the DVC of saline-injected (A) and LPS-treated (B) rats. C-D: c-Fos and nesfatin-1 (green) double-labeling in coronal sections through the DVC of LPS-treated rats. D: High magnification of c-Fos/nesfatin-1 positive neurons in the NTS. Dashed box in C indicates the area where high magnification image originates. Arrowheads: c-Fos+/Nesf+ neurons; arrow: c-Fos+ neuron negative for nesfatin-1. E: Quantification of the number of c-Fos and c-Fos/nesfatin-1 immunoreactive cells at the NTS subpostremal level of rats injected with either saline or LPS solution. Starred values are significantly different from saline-injected animals, *** p < 0.001. AP, area postrema; cc, central canal; DMNX, dorsal motor nucleus of the vagus; NTS, nucleus of the solitary tract; ts, tractus solitarius. Scale bars: 100 μm (A-C), 50 μm (D).