Table 2 PCR primer sequences
End-point PCR Primers | Forward | Reverse | Size (bp) |
|---|---|---|---|
GAPDH | ACCACCATGGAGAAGGCTGG | CTCAGTGTAGCCCAGGATGC | 509 |
IL-1β | ATG GCAGAAGTACCTGAGCTC | TTAGGAAGACACAAATTGCATGGTGAA | 810 |
IL-6 | GTGTGAAAGCAGCAAAGAGGC | CTGGAGGTACTCTAGGTATAC | 159 |
GFAP | CTGGGCTCAAGCAGTCTACC | CTGGGGTTAAGAAGCAGCAG | 666 |
GS | AAGTGTGTGGAAGAGTTGCC | TGCTCACCATGTCCATTATC | 234 |
S100β | ATGTCTGAGCTGGAGAAGG | CTGTCTGCTTTCTTGCATG | 415 |
Real-time PCR Primers | Forward | Reverse | Size (bp) |
β-actin | TCACCCACACTGTGCCCATCTACGA | CAGCGGAACCGCTCATTGCCAATGG | 295 |
Vimentin | GAGAACTTTGCCGTTGAAGC | TCCAGCAGCTTCCTGTAGGT | 170 |
IL1β | AAGGCGGCCAGGATATAACT | CCCTAGGGATTGAGTCCACA | 102 |
GFAP | TCTCTCGGAGTATCTGGGAACTG | TTCCCTTTCCTGTCTGAGTCTCA | 81 |