Figure 5
Confocal micrographs of double-labeling for CCR2 and GFAP in dentate gyrus and CA1. In the dentate gyrus of SE rats (D-F) the double-labeling is present in the radial processes that extend through the granular cell layer (white arrowheads in F). In addition, a relatively large population of CCR2-labeled astrocytes is oriented toward the hilus (white arrows in F). Alternatively, control rats (A-C) presented CCR2 staining only in the cell body (black arrow in C). This labeling pattern is also observed in SE rats (black arrows in F). In the CA1region of SE rats (J-L) there is a robust co-localization of CCR2 and GFAP in many astrocytes (black arrowheads in L). Note that many of these double-labeled astrocytes have a hypertrophied appearance. In the CA1 region of control rats (G-I), the CCR2 labeling is very limited, being restricted to the cell body (black arrow in I). CCR2 staining in cell bodies (black arrows) of control and SE rats was not observed to co-localize with GFAP. Scale bar: dentate gyrus, 40 Ī¼m; CA1, 20 Ī¼m.