Figure 4

Neuroprotection following media exchange between mixed and neuronal-enriched cultures. Media from mixed and neuronal-enriched (NE) cultures was exchanged (see methods) prior to challenge with NMDA (average [NMDA] = 15 μM for NE cultures and 20 μM for mixed cultures) in the presence or absence of the EP1 antagonist SC51089 (shown as SC). The source of the media is shown above the bars and the culture below the bars. This is the average of four independent experiments. The protection by SC in the neuronal-enriched cultures with mixed media was significant (P < 0.01) by ANOVA, Tukey-Kramer multiple comparison test. The results shown were then averaged from four independent experiments.