Impact of iron on MCP-1 secretion. Vector-transfected SH-SY5Y cells (A) and SH-SY5Y cells expressing Wt HFE (B), H63D HFE (C), or C282Y HFE (D) were treated with 10 μM, 5 μM, or 1 μM desferroxamine (DFO) or 10 μM, 30 μM, or 90 μM ferric ammonium citrate (FAC) for 48 hours and the secretion of MCP-1 was determined by ELISA and normalized to cellular protein content. n = 4. *, **, and *** indicate p < 0.05, 0.01, and 0.001 compared to control. NS = not significant.