Figure 6

GSK3 inhibition attenuates IL-6 production and astrogliosis. Mice were fed for 3 weeks without or with lithium chow. A, Serum and brain region homogenate (100 μg protein) levels of IL-6 were measured 18 h after ip injection of LPS or saline vehicle control (C) to evaluate the relative long-term outcomes of acute sepsis (n = 4–6 mice/group, *p < 0.05, compared to mice injected with LPS without lithium). B, Activation of STAT3 was measured by immunoblotting phospho-Tyr705-STAT3 in brain regions 18 h after ip injection of LPS. Means ± SEM (n = 4–6 mice/group, *p < 0.05 compared to mice injected with LPS without lithium). C, GFAP in brain regions 18 h after LPS (ip). Means ± SEM (n = 4–6 mice/group,*p < 0.05, compared to mice injected with LPS without lithium).