a): Confocal microscopy images of ischemic middle cerebral artery (MCA) labeled for pERK 1/2 (A-C), pp38 (D-F) and pJNK (G-I). There was increased pERK 1/2 in MCA of the MCAO group as compared with control, and treatment with U0126 at 0 hour prevented this increase. Activation of pp38 and pJNK were not changed in the MCAO and treatment groups. Scale bar 50 μm. b): Bar graphs showing fluorescence intensity for pERK1/2, pp38 and pJNK in MCA. Activation of pERK1/2 was significantly increased in MCAO as compared to control group. Treatment with U0126 significantly decreased this activation. There was no alteration in protein expression of pp38 and pJNK in the control, MCAO and treated groups. Data are presented as the mean percentage relative to control ± SEM.; n = 5. *P < 0.05, significant difference between control group and MCAO, #P < 0.05, significant difference between MCAO and treated group.