Figure 4
From: Reduction of β-amyloid pathology by celastrol in a transgenic mouse model of Alzheimer's disease
A) Representative western-blot depicting the effect of celastrol on BACE-1 expression in HEK293 cells overexpressing APPsw. Celastrol inhibited basal BACE-1 expression as well as the stimulation of BACE-1 expression induced by a 24 hours treatment with PMA. APP-CTFβ level is reduced when BACE-1 expression is inhibited by celastrol and APP-CTFβ level is increased when BACE-1 expression is stimulated by PMA. B) Histogram representing the quantification of BACE-1 expression in response to celastrol and PMA treatments. ANOVA reveals a significant main effect of PMA (P < 0.002) and of celastrol (P < 0.001) on BACE-1 expression. Post-hoc comparisons shows that PMA significantly stimulates BACE-1 level (P < 0.002) whereas celastrol significantly reduces BACE-1 expression (P < 0.001) compared to control condition and opposes BACE-1 stimulation induced by PMA (P < 0.001). (** P < 0.002).