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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Norepinephrine enhances the LPS-induced expression of COX-2 and secretion of PGE2 in primary rat microglia

Figure 4

Effect of different α- and β-adrenergic agonists and antagonists on COX-2 protein expression. Microglial cells were stimulated with LPS 10 ng/ml and different adrenergic agonists/antagonists for 4 hours, followed by western blot analysis for COX-2 protein expression (70 kDa). (A) LPS-stimulated microglial cells were treated with different α- and β-adrenoreceptor agonists at two different concentrations (1 and 10 μM): α1-agonist phenylephrine; α2-agonist clonidine; β12-agonist dobutamine; β2-agonist terbutaline. (B) LPS-stimulated microglial cells were treated with different α- and β-adrenoreceptor antagonists at two different concentrations (1 and 10 μM): α12-antagonist nicergoline; β1-antagonist CGP 20712A; β2-antagonist ICI118, 551. (C) LPS-stimulated microglial cells were treated both with dobutamine or terbutaline and with the two different β-adrenoreceptor antagonists ICI 118,551 or CGP 20712A. [NE = norepinephrine].

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