Figure 10

Double staining of cerebellum tissue sections with a commercial antibody to p80-coilin identifies the dotted nuclear staining seen with the broad majority of cerebellar neurons following incubation with the patient's serum (but not CSF) as anti-coilin immunoreactivity. The patient's antibody is depicted in green (A; AF488); the commercial coilin antibody in red (B; AF568); yellow color indicates overlay of the two antibodies (C). The p80-coilin antibody recognized a ~80 kDa protein band (lane 1) in a western blot of primate cerebellum tissue, which was not identical to the 80-97 kDa band found with the patient's serum and CSF (lane 2).