Figure 13

Double labeling of a primate cerebellum tissue section with a commercial antibody to inositol-triphosphate receptor I (IP3RI), depicted in red (AF568), and the patient's CSF, shown in green (AF488). Nuclei are shown in blue (DAPI). An almost perfect overlay (yellow) of the two antibodies was found in the molecular layer and the Purkinje cell (PC) layer (Panels A-C), indicating that the patient's antibody targets an antigen expressed in close spatial proximity of IP3RI. Higher magnification (40×; D) suggests that the two antibodies might also bind to dendritic spines of PCs, though not all spines were stained by both antibodies. A fair, though less impressive, overlay was found in the white matter, where the two antibodies stained the same axons (E-G).