Synaptophysin immunolabeling in spinal cord ventral horn. Normal synaptophysin immunoreactivity on the side contralateral to the lesion (A). Observe the less intense labeling in the ipsilateral side after the avulsion alone (B); synaptic labeling is greater if avulsion is combined with EAE (C and D). (E) Graph representing quantification of immunolabeling for all groups. (* = p < 0.05). Scale bar = 50 μm.