Figure 3
From: Herpes simplex virus induces neural oxidative damage via microglial cell Toll-like receptor-2
HSV-infected microglial cells from TLR2-/- mice produce less neural oxidative damage than wild-type microglia when added to mixed brain cultures. Control or HSV-infected (MOI = 2) purified murine microglia from wild-type and TLR2-/- were transferred at concentrations of 5 × 104 or 1 × 105 to wells containing a mixed neural culture. Cultures were harvested 48 h after microglial cell transfer and assessed for 8-isoprostane production. Data are presented as mean pg/mL of 8-isoprostane ± SD and are a representative of three separate experiments.