Brain damage and cell death after traumatic brain injury in wild type (Wt) mice and gp91phox-/- mice. (A) 2,3,5-triphenyltetrazolium chloride (TTC)-stained coronal brain sections from Wt mice (left) and gp91phox-/- mice (right) 2 days after TBI. (B) The contusion area in gp91phox-/- mice (170.5 ± 71.5 mm2 × 10-2, n = 20) was significantly smaller than that in the Wt group (290.7 ± 94.0 mm2 × 10-2, n = 10, *p < 0.01, t test). (C) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-staining in peri-contusional area of wild type (left) and gp91phox-/- (right) mice 48 hours after TBI. TUNEL-positive cells numbers in the gp91phox-/- mice (117 ± 69.3 cells/area, n = 6) were significantly smaller than those in the Wt group (252 ± 128.9 cells/area, n = 6, *p < 0.05, t test) (D). All values represent the mean ± SE.