Oxidative stress is greater in cPLA
α+/+ than in cPLA
α-/- mice after MCAO. A: ROS were measured in cPLA2α+/+ and cPLA2α-/- mice that were injected with dihydroethidium (HE) immediately before MCAO or after sham surgery. The intensity of HE staining in brain sections from the indicated time points was measured by densitometry. Ipsi, ipsilateral; Contra, contralateral; n = 3 mice/condition with 120-130 neurons counted/mouse; † P < 0.01. B: CBF was measured in mice by [14C]-IAP autoradiography in the ACA and MCA territories of each cortex at 60 minutes of MCAO. The left axis shows the position (in mm) of each region relative to bregma, and the right axis shows the absolute rCBF (ml/100 g/min); 24 slices from n = 4 mice/group. * P < 0.01 and † P < 0.05 as compared to the same region in cPLA2α+/+ mice.