Figure 3

In control slices without TNFα pretreatment, OLDA application did not induce a significant change in mEPSC frequency. Incubation with the cytokine TNFα increased basal mEPSC frequency (n = 10; *P < 0.05) in a population of DH neurons with capsaicin-sensitive primary afferent fibre inputs, when compared to recordings from control slices without TNFα incubation (n = 25). The high statistical significance of the mEPSC frequency increase during OLDA application in neurons after TNFα incubation was also evident without data normalization (**P < 0.01).