Figure 8

Regulation of TNF-α and CXCL10 production. Culture supernatants collected from human astrocytes pretreated with (B, D) or without (A, C) SnPP (10 μM) for 3 h prior to hemin (10 and 20 μM) treatment for 24 h followed by IL-1β exposure were measured for A, B) TNF-α (24 h) or C, D) CXCL10 (48 h) by ELISA. Data presented are mean ± SE of triplicates from 2 separate experiments using different brain tissue specimens. ** p < 0.01 vs. untreated control (C); ^†p < 0.05 and ^††p < 0.01 vs. IL-1β alone; ^§p < 0.05 and ^§§p < 0.01 vs. corresponding hemin+IL-1β.