Figure 6

Neuropeptides positively regulate microglial migration. The effect of neuropeptides on migration of BV2 (A, C, E) and N9 (B, D, F) cells were analyzed in a transwell assay after 3 h incubation with increasing concentrations of BK (A, B), SST (C, D) or ET (E, F). Cells that passed through the membrane were harvested, stained with calcein AM dye, and fluorescence was read at 485 nm excitation and 520 nm emission. (n = 4 +/- SEM; one-way ANOVA followed by Tukey's post test. ** P < 0.01 vs. control. *** P < 0.001 vs. control.). Similar results were obtained in 2 additional experiments for BV2 and N9 cells.